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Library Dissertation Conservative Dentistry

This collection includes theses and dissertations from the School of Dentistry. It is not exhaustive since most paper theses and dissertations have not been digitized. Generally, paper copies of theses and dissertations published prior to 2014 can be accessed in the University of Louisville Libraries. If you would like to add your thesis or dissertation to this collection, please see: http://ir.library.louisville.edu/about.html.

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Theses/Dissertations from 2017

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Inhibition of chemotherapeutic-induced apoptosis in esophageal squamous cancer cells by the keystone periodontal pathogen, Porphyromonas gingivalis., Atul Kumar Agrawal

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Expression of ZEB factors in oral squamous cell carcinoma., Saira Ahmed

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Using an audience response system to calibrate dental faculty assessing student clinical competence., Sean A. Aiken

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Student self-assessment of operative dentistry experiences : a time dependent exercise in best practice outcomes., Celine Joyce Cornelius Timothius

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Treg regulation in periodontal disease during pregnancy., Aislinn Leah Hays

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Function of the enterobactin operon of A. actinomycetemcomitans in the presence of catecholmines and iron., Taylor Johnson

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Deficiency of RDH10 causes cleft secondary palate in mouse., Swetha Raja

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Exploring a novel NF-ĸB- inhibiting nanoparticle for periodontitis therapy., Kameswara Satya Srikanth Upadhyayula

Theses/Dissertations from 2016

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Retinoic acid signaling regulates KRT5 independently of stem cell markers in submandibular salivary gland epithelium., Timur Maratovich Abashev

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Objective evaluation of clinical shade-matching outcomes., Erin Ballard

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Tobacco-gene activity profile in Porphyromonas gingivalis, Filifactor alocis and Treponema denticola., Neelima Chowdary Cherukumalli

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Modification of T9SS cargo proteins of Porphyromonas gingivalis for their secretion in soluble form and purification by affinity chromatography., Apoorv Goel

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Cannabinoids suppress the innate immune response to periodontal pathogen Porphyromonas gingivalis in gingival epithelial cells., Rajarshi Guha Niyogi

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Mechanism of interaction of peptide modified nanoparticles with Porphyromonas gingivalis., Ankita Jain

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Peptoanaerobacter stomatis interactions with neutrophils., Emeri Jimenez Flores

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Long term facial alveolar bone changes associated with endosseous implants in the anterior maxilla., Deepika Joshi

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Influence of in-office oral hygiene instructions on the parental assessment of their child's oral health., Sireesha Kodali

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Porphyromonas gingivalis gingipains induce a pro-inflammatory extracellular microenvironment : the role of PAR-2 and fibronectin., Jeffrey S. Marschall

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Characterization of PG1604 (PorZ) as an essential component of type 9 secretion system in Porphyromonas gingivalis., Apurva Tadimari Prabhakar

Theses/Dissertations from 2015

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Vitamin A is essential for proper embryonic submandibular salivary gland growth., Deanna Ellene Buenger

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Evaluating the marginal integrity of lithium disilicate veneers fabricated by digital impressions and CAD/CAM compared to conventional techniques., Michael C. Guzelian

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Synthesis and functional evaluation of peptide modified poly (lactic-co-glycolic acid) nanoparticles to inhibit porphyromonas gingivalis biofilm formation., Paridhi Kalia

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Comparison of digital scanning and polyvinyl siloxane impression techniques by DMD students : instructional efficiency and attitudes toward technology., Alexander M. Marti

Theses/Dissertations from 2014

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Effect of metallic restoration artifacts on maxillofacial cone beam computed tomography images., Mitali Binani 1987-

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Cholinergic inhibition of lipopolysaccharide induced matrix metalloproteinase in immune cells., Harrison Black, 1988-

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Neutrophils alter epithelial response to Porphyromonas gingivalis in a gingival crevice model., Jessica Lynn Bondy-Carey 1987-

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Clinical efficacy of cross-sectional imaging compared with panoramic imaging and virtual 3D models for the assessment of dental implant placement., Moiz Ahmad Khan 1987-

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The role of Porphyromonas gingivalis in the regulation of epithelial-mesenchymal transition markers in migration., Akintunde Ojo 1981-

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Interleukin-6 production and epithelial cell signaling by telomerase immortalized gingival keratinocytes in Porphyromonas gingivalis infection., Himabindu Vuddaraju 1988-

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Implementation of an oral health intervention for adults with intellectual and developmental disabilities., Brigit Rene Zaksek 1974-

Theses/Dissertations from 2013

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The dimerization of parotid secretory protein., Diana M. Blau

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Cigarette smoke promotes genomic evolution in the periodontal pathogen Porphyromonas gingivalis., Josh George

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Sexual dimorphism in periodontitis in a mouse model., Robert C. Gleason 1988-

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Peptide mediated inhibition of Porphyromonas gingivalis dual and three species biofilms., Naga Srinija Gummadi

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Characterization of promoter regions of parotid gland genes., Sara M. Perez 1987-

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XBP1 and MIST1 are not sufficient to induce the regulated secretory pathway in parotid cell lines., Ian S. Pyle 1986-

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Comparison of marginal fit of lithium disilicate crowns fabricated with CAD-CAM technology using conventional impressions and two intra-oral digital scanners., Kelly E. Rogers

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Genetic characterization of the K2 serotype capsule of Klebsiella pneumoniae ATCC 43816 and the development of a bioluminescent strain., Jacob Brandon Scott 1987-

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Patient preference and compliance between Hawley retainers and vacuum-formed retainers following orthodontic treatment., Justin Wild

Theses/Dissertations from 2012

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Using a statistical model to locate orthodontic underserved areas capable of supporting practices in Kentucky., Wes Cardall

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Effectiveness of fluoride containing bonding resins in preventing demineralization of enamel during orthodontic treatment., Robin J. Choi

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Iron acquisition by the dental pathogen aggregatibacter actinomycetemcomitans., Porscha LaRai Jefferson 1986-

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Click scaffolds for the inhibition of Porphyromonas gingivalis and Streptococcus gordonii biofilm formation., Catherine Marie Loner

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The effect of implant placement and simultaneous soft tissue augmentation in the esthetic zone using either connective tissue autograft or acellular dermal matrix allograft., Thomas L. Peterson 1980-

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Identifying Aggregatibacter actinomycetemcomitans periodontal antigens by immunoscreening., Gerald Bernard Pevow

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Ridge preservation comparing the clinical and histologic healing of an intrasocket allograft vs. a facial overlay xenograft using a bioresorbable barrier membrane., Evmenios Poulias

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Effect of viewing conditions on the detection of proximal dental caries in intraoral digital images with and without computer-assisted diagnosis., Lauren Christine Szechy 1989-

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The patient's view of orthodontic training requirements and the influence of board certification or a master's degree on their choice for an orthodontist., Justin Trisler 1980-

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The effect of implant placement and simultaneous soft tissue augmentation in the esthetic zone using either connective tissue autograft or acellular dermal matrix allograft on peri-implant hard and soft tissue healing., Gretchen A. Wigand

Theses/Dissertations from 2011

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Generational differences in perceived lip protrusion esthetics., David Butler 1979-

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Interproximal reducation in conjunction with plastic aligner therapy : a retrospective pilot study., Adam Chorak

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Age-associated periodontal bone loss in normal and TLR2- or MyD88-deficient mice., Paul A. Ciero 1986-

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Patient perspective post-orthognathic surgery., Jessica Tunstill Crews

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Comparison of two orthodontic indirect bonding methods., Gary D. Dixon

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Root coverage comparing acellular dermal matrix to connective tissue graft using the coronally positioned tunnel technique., Marie-Eve Girouard

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Ridge preservation comparing the clinical and histologic healing of a demineralized particulate bone allograft vs. mineralized particulate cancellous allograft using barrier membrane., Veneta Kotevska

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A survey study comparing adult orthodontic patient quality of life between Invisalign and fixed appliances., Keith Christopher Nicholson

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Contribution of the Rv1985c transcriptional regulator to Mycobacterium tuberculosis intracellular adaptation., Nicholas B. Wantland 1984-

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Incidence of soft tissue calcifications of the head and neck region on maxillofacial cone beam computed tomography., Adam B. Wells 1977-

Theses/Dissertations from 2010

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Presence/absence of an endodontic specialty program during dental education compared to general dentist attitudes toward treating or referring patients requiring endodontic therapy., Gregory A. Carman 1967-

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De-differentiation of parotid acinar cells in vitro., Andrew Scott Currie 1983-

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Direct binding of parotid secretory protein to phosphatidylinositol phosphates., Dipti Goyal 1984-

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Effect of finger rings on microbial cross-contamination during dental clinical procedures., Swapna Nadikuda

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Ridge preservation comparing the clinical and histologic healing of a mineralized cortical vs. mineralized cancellous allograft with an acellular dermal matrix barrier membrane., Marquez J. Sams

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In vitro detection of mesio-buccal canals in maxillary molar cross-sections using three different resolutions with Kodak 9000 3D CBCT., Jolanta Nowicka Sauer

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The effect of the blue light diode laser on shear-peel bond strength of orthodontic brackets with varying curing time and distance., Kamran Shaikh 1978-

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A comparison of the effectiveness of two suturing techniques for the coronally positioned tunnel procedure with an acellular dermal matrix allograft., Courtney Tyler Shearer

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Comparison of clinically projected and radiographically optimal implant fixture placement., William Shane Vaughn 1984-

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3D CBCT analysis of cranial base morphology and its relationship to skeletal malocclusion., Russell M. Weaver 1973-

Theses/Dissertations from 2009

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Ridge preservation comparing the clinical and histologic healing of a mineralized particulate allograft with a nonporous PTFE membrane vs. mineralized particulate xenograft with a collagen plug membrane., Jason A. Witonsky

Theses/Dissertations from 2008

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Altered developmental programming of the mouse mammary gland in female offspring following perinatal dietary exposures : a systems-biology perspective., Caleb Deen Bastian

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Comparative linear accuracy of cone beam CT derived 3D images in orthodontic analysis., April A. Brown 1983-

Theses/Dissertations from 2007

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Comparative linear accuracy and reliability of cone beam CT derived 2-dimensional and 3-dimensional images constructed using an orthodontic volumetric rendering program., Danielle R. Periago

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Ridge preservation comparing a flap vs. a flapless technique using a demineralized bone matrix allograft plus mineralized particulate allograft and covered with a calcium sulfate barrier., Trever L. Siu

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Cone beam computed tomographic simulation of panoramic radiology : third molar assessment and mandibular canal., Ryan L. Snyder

Theses/Dissertations from 2006

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Comparative TMJ imaging accuracy using iCAT cone beam computerized tomography., Oana Bida Honey

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Implant esthetics using a Nobel Replace Standard implant vs. Nobel Perfect Scalloped implant placed in immediate extraction sockets in the esthetic zone of the maxilla., John W. McGehee

Theses/Dissertations from 2005

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A cross sectional analysis of the relationship between childhood obesity and craniofacial dimensions in a population receiving orthodontic treatment., Daniel Mark Eves

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Ridge augmentation comparing a cancellous block allograft to freeze dried bone particles and utilizing an acellular dermal matrix barrier membrane., Brenton Lahey

Theses/Dissertations from 2004

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The current crisis in orthodontic education : the residents' perspective., Matthew Kawabori Bruner

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A clinical comparison of decalcification following orthodontic treatment using composite resin and reinforced modified glass ionomer cements., Jennifer L. Butterfoss 1976-

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Cephalogram pseudo-color and emboss enhancements : anatomical landmark clarity in photostimuable phosphor cephalometrics., Ryan B. Wiesemann 1977-

 

INTRODUCTION: Historically, treatment protocols for infected, immature teeth involved disinfection of the root canal space (RCS) followed by creation of an apical barrier and subsequent obturation.  While generally effective at removing the source of infection, these methods resulted in teeth with thin, underdeveloped roots that were prone to fracture over time.                                           

An alternative to the conventional methods is now available in the form of regenerative endodontic procedures.  These are currently performed to treat infected immature teeth utilizing the “revascularization method.” This method includes two steps: disinfection of the root canal followed by the laceration of apical tissues to induce blood flow into the root canal space.  The blood clot formed in the canal serves as a fibrin scaffold for the accumulation of stem cells and other cells with dentin/root formation capability.  This protocol has resulted in continued root formation (in length and width) in certain clinical cases.  However, the adequacy of hemorrhage induced in the apical tissues has a significant impact on the quality of the regenerated dental structures.  This phenomenon indicates that the blood clot itself may play an important role in continued root formation by locally releasing molecular signals, which induce angiogenesis.  Successful angiogenesis is then crucial for the vitality of the newly regenerated pulp tissues and for the initiation of continued root development.  Therefore, the production of angiogenic growth factors from the local surviving cells or the effective delivery of exogenous angiogenic growth factors to the desired area is one of the key components in regenerative endodontic treatment.                 

 It is believed that an appropriate exogenous scaffold could aid the endogenous elements (fibrin meshwork and molecular signals in blood clot) and result in more predictable regenerative endodontic procedures.  DynaMatrix® is a biological extracellular matrix (ECM) scaffold product that is marketed to dental practitioners.  In addition to structural and functional proteins (collagens types I, III, IV, and VI, glycosaminoglycans, glycoproteins, proteoglycans) DynaMatrix contains biological signals such as cytokines.  Its track record of successful use in periodontal procedures encourages investigation of the material for use in regenerative endodontic procedures.  Specifically, DynaMatrix may be placed within the root canal system of the immature tooth to facilitate the continued development of the entire length of the root from the apical foramen to the level of the Dentin Enamel Junction (DEJ).  As a scaffold containing biological signals, DynaMatrix has the potential to induce angiogenesis and encourage the migration of cells that are involved in the continued development of the root canal system.                    

PURPOSE: The aim  was to determine if the exposure of human dental pulp stem cells (HDPSC) to the DynaMatrix membrane will result in an increased production of angiogenic growth factors that are critical for pulp/root regeneration. 

MATERIALS & METHODS:  This study utilized human dental pulp cells (HDPSC) in vitro.   Three different groups were tested as follows: (1) control group 1- Human Dental Pulp Cell (HDPC) culture only; (2) control group 2 – DynaMatrix®membranes placed in culture media without any cells present and (3) experimental group- HDPSC seeded on DynaMatrix® membranes.  The conditioned media from the various groups was collected and tested for the expression of specific cytokines using angiogenesis cytokine arrays which have been established as a viable method for assessing expression of angiogenic cytokines.                            

To determine the level of cytokine expression, the optical densities of the visible dots on the membrane were measured by Quantity One 1-D Analysis Software (Bio-Rad). For each membrane, the density of each dot was adjusted for the background by subtracting the average value of the negative controls on each membrane and then normalized by dividing by the average of the positive controls.  The experiment was run three times resulting in three membranes for each group.  This generated 4-6 densitometry readings (two per membrane, some statistical outliers removed) for each cytokine in each group.  Group comparisons were performed using one-way ANOVA.

RESULTS: The cell-only group (C) had significantly lower ENA-78 (p=0.0166), leptin (p=0.0208), PDGF-BB (p=0.0034), VEGF (p=0.0174), VEGF-D (p=0.0230), and bFGF (p=0.0047) compared to the DynaMatrix membrane-only group (M). C had significantly higher IL-6 (p=0.0268), IL-8 (p=0.0153), MCP-1 (p=0.0021), TIMP-1 (p=0.0027), and TIMP-2 (p=0.0105) compared to M.

C had significantly lower PDGF-BB (p=0.0182) and bFGF (p=0.0009) compared to the DynaMatrix membrane + dental pulp stem cell group (M+C). Control had significantly higher IL-6 (p=0.0203), IL-8 (p=0.0097), MCP-1 (p=0.0019), TIMP-1 (p=0.0006), and TIMP-2 (p=0.0086) compared to M+C. C and M+C did not have significantly different ENA-78 (p=0.78), leptin (p=0.42), VEGF (p=0.42), or VEGF-D (p=0.07).

M had significantly lower bFGF (p=0.0452) compared to M+C. M had significantly higher ENA-78 (p=0.0178), TIMP-1 (p=0.0363), and VEGF (p=0.0117) compared to M+C. M and M+C did not have significantly different IL-6 (p=0.79), IL-8 (p=0.63), leptin (p=0.06), MCP-1 (p=0.92), PDGF-BB (p=0.10), TIMP-2 (p=0.81), or VEGF-D (p=0.34).                                            

CONCLUSION:  The results of this study demonstrate that HDPSC can grow successfully on DynaMatrix in vitro, and that DynaMatrix may contribute to success in regenerative endodontic procedures by providing a predictable mechanical scaffold for cellular and vascular in-growth into the RCS.  Additionally, it may improve angiogenesis by increasing the quantity of angiogenic growth factors present in the microenvironment via release of angiogenic cytokines present in DynaMatrix, as well as possible interactions with the cells involved in regeneration that result in greater expression of angiogenic cytokines from these cells.